Zh.V. Markina, A.A. Zinov - Population Growth of Microalga Tisochrysis lutea (Haptophyta) and the Content of Carotenoids and Neutral Lipids at the Different Illuminations in a Panel Bioreactor

BIOLOGIYA MORYA, 2025, Vol. 51, No. 1, pp. 20-27

Population Growth of Microalga Tisochrysis lutea (Haptophyta) and the Content of Carotenoids and Neutral Lipids at the Different Illuminations in a Panel Bioreactor

A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok 690041, Russia

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The effect of illumination (10, 30, 50, and 70 μmol/m²/s) on microalga Tisochrysis lutea (Haptophyta) in a 1.8 L panel bioreactor was studied using a 5 Lux-LED flat panel (Infors HT, Switzerland) for 14 days. It was found that the most intensive growth of microalga occurred at 30 and 50 μmol/m²/s. At 10 μmol/m²/s, population growth was completely suppressed. Regardless of the light intensity, cells larger than 4 μm predominated in the algae population after seven days. The carotenoid content at illuminations of 10 and 70 μmol/m²/s was highest on the seventh day. The chlorophyll a to carotenoid ratio decreased by the end of the experiment in all conditions. The highest amount of neutral lipids in cells was noted at 10 μmol/m²/s.

Key words: Tisochrysis lutea, illumination, bioreactor, carotenoids, neutral lipids.